recombinant noggin (rnoggin  (PeproTech)

Journal: iScience

Article Title: Identification of novel macrophages and bone morphogenetic protein signals causing ectopic calcification and impairing muscle regeneration

doi: 10.1016/j.isci.2025.112841

Figure Lengend Snippet: BMP signaling inhibition reduced calcification and promoted regeneration (A) Experimental protocol for analyzing animals treated with rNoggin or PBS. (B) mRNA expression levels of Id1 (3dpi) and Runx2 (5dpi) as detected by qPCR in NTX-injured muscles subsequently treated with PBS or rNoggin ( n = 4). (C) Alizarin Red and H.E. staining of TA muscles at 5 days post-NTX injury in WT mice treated with PBS or rNoggin. (D and E) Quantification of the area of calcium deposition and average CSA in NTX-injured muscles treated with PBS ( n = 4) or rNoggin at 5 dpi ( n = 5), respectively (3 images per mouse). (F) Quantitation of the relative expression of Myog by RT-qPCR in NTX-injured muscles treated with PBS or rNoggin at 5 dpi ( n = 4). (G) Experimental protocol for analyzing animals treated with LDN-193189 or vehicle. (H) mRNA expression levels of Id1 and Runx2 as detected by qPCR in NTX-injured muscles treated with vehicle or LDN-193189 at 3 dpi ( n = 4). (I) Alizarin Red and H.E staining of TA muscles at 5 days post-NTX injury in WT mice treated with either vehicle or LDN-193189. (J and K) Quantification of the area of calcium deposition and average CSA in NTX-injured muscles treated with vehicle or LDN-193189 at 5 dpi, respectively ( n = 5, 3 images per mouse). (L) Quantitation of the relative expression of Myog and Myh3 by RT-qPCR in NTX-injured muscles treated with vehicle or LDN-193189 at 5 dpi ( n = 4). (M) The Rotarod test shows the faster recovery of muscle function in mice treated with LDN-193189 ( n = 6/7). (N) The ratio of injured TA/uninjured TA/Quadriceps weight to BW at 12 dpi from mice treated with LDN-193189 ( n = 6). Data are expressed as the mean ± SD in all panels. ∗ p < 0.05, ∗∗ p < 0.01. Scale bars: in (C) and (I), upper, 200 μm; below, 50 μm. See also

Article Snippet: rNoggin was purchased from R&D Systems (1967-NG) and prepared as recommended.

Techniques: Inhibition, Expressing, Muscles, Staining, Quantitation Assay, Quantitative RT-PCR

Journal: iScience

Article Title: Identification of novel macrophages and bone morphogenetic protein signals causing ectopic calcification and impairing muscle regeneration

doi: 10.1016/j.isci.2025.112841

Figure Lengend Snippet: BMP signaling regulated ectopic calcification in Csf1r cKO mice (A) The expression of Id1 and Runx2 via qPCR at 5 days post BaCl 2 injury in Csf1r cKO mice treated with PBS and rNoggin ( n = 4). (B) Alizarin Red and H.E. staining of TA muscles at 5 days post BaCl 2 injury in Csf1r cKO mice treated with PBS or rNoggin. (C–E) Quantification of the area of calcium deposition and necrotic myofibers as well as average CSA in BaCl 2 -injured muscles in Csf1r cKO mice treated with PBS and rNoggin at 5 dpi, respectively ( n = 5, 3 images per mouse). (F) Quantitation of the relative expression of Myog by RT-qPCR in BaCl 2 -injured muscles from Csf1r cKO mice treated with PBS or rNoggin at 5 dpi ( n = 4). (G) The expression of Id1 and Runx2 via qPCR at 5 days post-BaCl 2 injury in Csf1r cKO mice treated with vehicle and LDN-193189 ( n = 4). (H) Alizarin Red and H.E. staining of TA muscles at 5 days post-BaCl 2 injury in Csf1r cKO mice treated with vehicle or LDN-193189. (I–K) Quantification of the areas of calcium deposition and necrotic myofibers as well as average CSA in BaCl 2 -injured muscles in Csf1r cKO mice treated with vehicle and LDN-193189 at 5 dpi, respectively ( n = 4, 3 images per mouse). (L) Quantitation of the relative expression of Myog by RT-qPCR in BaCl 2 -injured muscles from Csf1r cKO mice treated with vehicle or LDN-193189 at 5 dpi ( n = 4). Data are expressed as the mean ± SD in all panels. Scale bars: in (B) and (H), upper, 200 μm; below, 50 μm. See also

Article Snippet: rNoggin was purchased from R&D Systems (1967-NG) and prepared as recommended.

Techniques: Expressing, Staining, Muscles, Quantitation Assay, Quantitative RT-PCR